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Thio-engineered antibodies represent a promising new approach to antibody engineering that has the potential to improve the efficacy and specificity of therapeutic and diagnostic agents. BOC Sciences is a leading provider of thio-engineered antibody services, offering a comprehensive range of services for antibody design, expression, purification, and conjugation. Our thio-engineered antibody services offer several advantages over traditional antibody engineering services, including increased stability and specificity, improved pharmacokinetics and half-life, and site-specific conjugation to a range of molecules.
Antibodies that have been changed to form a thioether bond with their target antigen are known as thio-engineered antibodies. This change is made by including a cysteine residue in the antibody molecule. This cysteine residue can then interact with an antigen's thiol group to produce a thioether link. Compared to conventional antibodies, thio-engineered antibodies provide a number of benefits. First, compared to the non-covalent interactions that usually bind antibodies and antigens together, the thioether bond is more durable. Higher affinity and specificity for the target antigen may be the result of this improved stability. Second, the pharmacokinetics and in vivo half-life of thio-engineered antibodies can be enhanced because they are less prone to proteolytic destruction. Better treatment effectiveness and less frequent doses can result from this. Finally, thio-engineered antibodies can be used for site-specific conjugation to other molecules, such as drugs or imaging agents. This can improve the specificity and efficacy of these molecules, while reducing off-target effects.
Fig. 1. Details of ADC construction (Chem. Soc. Rev., 2022, 51, 3886-3897).
ADCs are a sort of targeted cancer therapy that combines the cytotoxicity of a medication with the specificity of an antibody. When an ADC binds to the target antigen on cancer cells, a cytotoxic drug molecule that is conjugated to the antibody is released. Thio-engineered antibodies are utilized to provide site-specific conjugation to the drug molecule, enhancing the efficacy and safety of ADCs. This makes it possible to precisely adjust the drug-antibody ratio (DAR), which can have an impact on the ADC's potency and toxicity. Thio-engineered antibodies have also been demonstrated to enhance the pharmacokinetics and stability of ADCs, which may result in increased therapeutic efficacy and less frequent dosage.
BOC Sciences is a top provider of biotechnology products and services, including designing and producing antibodies. The whole range of thio-engineered antibody services provided by us includes antibody design, expression, purification, and conjugation. The capabilities of thio-engineered antibodies from BOC Sciences include:
BOC Sciences has a team of experienced antibody engineers who can design thio-engineered antibodies based on specific customer requirements. This includes selecting the appropriate cysteine residue for thioether formation, optimizing the antibody structure for stability and specificity, and designing site-specific conjugation sites for drug or imaging agent conjugation.
BOC Sciences offers a range of expression systems for thio-engineered antibody production, including bacterial, mammalian, and insect cell expression systems. Our expression systems are optimized for high yield and quality, and can be customized to meet specific customer requirements.
BOC Sciences offers a range of purification techniques for thio-engineered antibodies, including affinity chromatography, ion exchange chromatography, and size exclusion chromatography. BOC Sciences' purification techniques are optimized for high purity and yield, and can be customized to meet specific customer requirements.
BOC Sciences offers site-specific conjugation of thio-engineered antibodies to a range of molecules, including drugs and imaging agents. BOC Sciences' conjugation techniques are optimized for high efficiency and specificity, and can be customized to meet specific customer requirements.
BOC Sciences, a leader in custom ADC conjugation services, can be contacted by researchers and drug developers for the design and manufacturing of high-quality ADCs for preclinical trials. Our company offers a wide range of services, including mAb manufacture, linker synthesis, drug conjugation, and analytical characterization, to facilitate the development of ADCs from concept to preclinical stages. Our ADC conjugation services are tailored to meet the specific needs of each project and can be customized to accommodate different antibodies, linkers, and drugs.
