With a strong foundation in synthetic chemistry and biotechnology, BOC Sciences supports end-to-end services covering antibody screening, linker design, drug payload preparation, and optimization of conjugation strategies, meeting the diverse needs at different stages of R&D. The platform is equipped with advanced analytical instruments and a production environment that meets quality standards, enabling processes from small-scale development to GMP-grade pilot and scale-up production, ensuring excellent product stability, safety, and consistency. We place particular emphasis on customized client requirements and offer modular, scalable project management with high responsiveness and scientific rigor to accelerate the development and translation of ADC projects.
Click reactions are highly adaptable to solvents, aqueous environments, and temperature, making them especially suitable for the modification of biomolecules like proteins, without requiring prior protection or activation of other functional groups.
Enables site-specific conjugation or control over the number of drug molecules conjugated, which is beneficial for optimizing the pharmacokinetic properties and therapeutic window of ADCs.
Multiple types of click reactions are available, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC), strain-promoted azide-alkyne cycloaddition (SPAAC), offering high selectivity and adaptability for various ADC design requirements.
Click linkers are chemically stable and resistant to hydrolysis, significantly enhancing the in vivo stability and targeted drug release of ADCs.
In ADC construction, click chemistry provides an ideal pathway for precise conjugation due to its high selectivity, bioorthogonality, and operational simplicity. BOC Sciences integrates click chemistry into multiple key stages of antibody-drug conjugation. By precisely controlling reaction sites, drug-to-antibody ratios, and conjugation methods, we ensure ADC uniformity and functional optimization. We develop multi-dimensional click chemistry strategies tailored to the characteristics of target molecules and clinical requirements, empowering innovation throughout the entire ADC development cycle—from early screening to clinical progression.
Enzyme-based or genetic engineering approaches are used to introduce alkyne/azide modification sites on antibodies, enabling highly precise site-specific conjugation to ensure product consistency.
Antibodies, linkers, and toxins are modified with corresponding functional groups and "assembled" via click reactions, enabling rapid screening and high-throughput ADC construction.
SPAAC and IEDDA systems are prioritized to minimize protein contamination and reduce purification burden, enhancing biosafety.
By tuning linker designs (e.g., ester bonds, disulfide bonds, self-immolative structures), we ensure in vivo stability while achieving efficient drug release in the tumor microenvironment.
Through controlled modification sites and reaction conditions, fixed DAR (e.g., 2, 4, 8) products are constructed to optimize the in vivo distribution and efficacy of ADCs.
BOC Sciences deeply understands the unique advantages of click chemistry in bioorthogonal reactions and has long been committed to the development and optimization of various click systems to meet the high-efficiency conjugation needs between antibodies, drug payloads, and linkers. By integrating cutting-edge technologies and extensive project experience, we have established multiple click chemistry platforms for ADC development, covering both classical and novel reaction mechanisms to ensure optimal results in conjugation efficiency, site specificity, and product purity. BOC Sciences provides customized click reaction design and implementation strategies to support the high-quality, controlled construction of ADCs, offering robust solutions for the precise crosslinking of complex drug molecules.
From toxin and linker customization, antibody modification, click conjugation, analytical verification, to GMP-scale production, BOC Sciences offers a complete technical cycle from laboratory research to clinical development.
We maintain a broad inventory of functionalized reagents including alkynes (–C≡CH), azides (–N₃), and strained alkynes (e.g., DBCO, BCN), supporting the development of various click chemistry pathways.
Whether for small-scale screening, process development, or pilot and GMP-scale production, BOC Sciences provides a complete set of reaction platforms and supporting facilities. Our conjugation reactors enable precise and scalable implementation.
We design personalized conjugation strategies based on client molecular structures, utilizing the orthogonality of click reaction sites to construct site-specific or dual-site ADCs, significantly enhancing product consistency and pharmacokinetic profiles.
Click conjugation is a technique that utilizes click chemistry to efficiently link two molecules, often used for the stable attachment of biomolecules like antibodies to small-molecule drugs. This method offers high selectivity, mild reaction conditions, and high yields, making it widely applicable in the construction of complex drug systems such as antibody-drug conjugates (ADCs).
The click chemistry process refers to the use of fast, efficient, and highly selective chemical reactions (e.g., copper-catalyzed azide-alkyne cycloaddition, strain-promoted reactions) to couple two functional molecules. These reactions typically do not require complex purification steps, proceed under mild conditions, and are suitable for molecular construction in organic, inorganic, or biological systems.
Click chemistry is highly favored due to its high reaction efficiency, strong specificity, and excellent biocompatibility. It typically avoids cross-reactivity with natural functional groups in biological systems, making it ideal for precisely connecting target molecules in complex environments, especially in drug development and biochemical labeling.
Click chemistry encompasses a class of efficient, selective, and mild conjugation reactions commonly used for constructing complex molecular structures. Bioorthogonal chemistry refers to chemical reactions that occur without interfering with natural cellular metabolism. Certain click reactions (e.g., strain-promoted azide-alkyne reactions) are typical examples of bioorthogonal reactions and are widely used in live-cell labeling and ADC construction.
