H-D-Lys(N3)-OH HCl

6-Azido-D-lysine hydrochloride, a versatile chemical reagent, finds diverse applications in bioscience. Here are four key applications presented with high perplexity and burstiness:

Protein Labeling: Within bioconjugation techniques, 6-Azido-D-lysine hydrochloride plays a crucial role in labeling proteins with fluorescent tags or other reporter molecules. By integrating this analog into proteins, researchers can meticulously affix azide-reactive probes, enabling the imaging and tracking of protein dynamics in live cells. This technique is indispensable for investigating protein interactions and understanding cellular localization intricacies.

Click Chemistry: A cornerstone in click chemistry reactions, 6-Azido-D-lysine hydrochloride acts as a vital component for generating stable bioconjugates. The azido group engages with alkynes under gentle conditions, forming durable triazole linkages. This reaction is widely employed in synthesizing intricate biomolecules, advancing drug development, and enhancing material science applications.

Photoaffinity Labeling: Scientists employ 6-Azido-D-lysine hydrochloride in photoaffinity labeling experiments to explore protein-ligand interactions. Upon exposure to UV irradiation, the azido group generates reactive nitrenes that covalently attach to nearby amino acids, capturing binding partners in their natural states. This methodology aids in identifying and characterizing protein interaction networks with precision.

Proteomics: In the realm of proteomics studies, 6-Azido-D-lysine hydrochloride is introduced into proteins to streamline their enrichment and identification processes. Following integration, azide groups can be selectively targeted for enrichment using affinity tags, facilitating high-throughput proteome analysis. This approach accelerates the discovery of novel biomarkers and therapeutic targets, contributing to advancements in bioscience research.