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CAS No.:
Cat No.: BADC-01249
Purity: 98.0%
4.5 
DBCO-PEG4-HyNic is a dual-reactive ADC linker with DBCO for SPAAC click and HyNic for oxime ligation, enabling stable, site-specific payload conjugation in antibody-drug conjugates.
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Popular Publications Citing BOC Sciences Products
DBCO-PEG4-HyNic is a versatile crosslinker used in bioconjugation, particularly for linking biomolecules in various biotechnological and pharmaceutical applications. Here are some key applications of DBCO-PEG4-HyNic:
Antibody-Drug Conjugates (ADCs): DBCO-PEG4-HyNic is used to conjugate antibodies to cytotoxic drugs, forming Antibody-Drug Conjugates. This approach enhances targeted cancer therapy by delivering cytotoxic drugs specifically to cancer cells while sparing healthy cells. DBCO-PEG4-HyNic enables stable and efficient linking, ensuring the therapeutic efficacy and safety of ADCs.
Protein Labeling: Scientists use DBCO-PEG4-HyNic for labeling proteins with various tags or probes, such as fluorescent dyes or affinity ligands. This technique is crucial for studying protein interactions, localization, and function in different cellular contexts. The biocompatible nature of DBCO-PEG4-HyNic ensures the biological activity of target proteins is preserved during the labeling process.
Surface Immobilization: DBCO-PEG4-HyNic is employed to immobilize biomolecules onto solid surfaces, such as biosensors or microarray platforms. By covalently linking biomolecules to these surfaces, researchers can develop highly sensitive diagnostic tools and analytical devices. The PEGylation aspect of DBCO-PEG4-HyNic further minimizes nonspecific binding and enhances the stability of immobilized biomolecules.
Drug Delivery Systems: In the field of drug delivery, DBCO-PEG4-HyNic is used to attach therapeutic molecules to nanoparticles or liposomes. This bioconjugation improves the targeted delivery and controlled release of drugs, enhancing their therapeutic potential and reducing side effects. The hydrophilic PEG (polyethylene glycol) spacer ensures better solubility and circulation properties of the conjugates in the bloodstream.
| Catalog | Product Name | CAS | Inquiry |
|---|---|---|---|
| BADC-01010 | DBCO-PEG4-amine | 1840886-10-3 | |
| BADC-01017 | DBCO-PEG5-acid | 1870899-46-9 | |
| BADC-01536 | DBCO-Peg4-VC-PAB-MMAE | 2129164-91-4 | |
| BADC-01073 | DBCO-PEG5-NHS ester | 2144395-59-3 | |
| BADC-01077 | DBCO-PEG4-DBCO | 2182601-68-7 | |
| BADC-00863 | DBCO-PEG4-Val-Cit-PAB-MMAF | 2244602-23-9 | |
| BADC-01089 | DBCO-PEG3 acetic-EVCit-PAB | 2253947-17-8 | |
| BADC-00868 | DBCO-(PEG2-VC-PAB-MMAE)2 | 2259318-55-1 | |
| BADC-00762 | DBCO-PEG4-VC-PAB-DMEA-PNU159682 | 2259318-56-2 | |
| BADC-00751 | DBCO-PEG4-MMAF | 2360411-65-8 |
What is the function of DBCO-PEG4-HyNic in ADC construction?
DBCO-PEG4-HyNic is a bioorthogonal linker enabling strain-promoted azide-alkyne cycloaddition (SPAAC). It connects antibodies to azide-modified payloads without requiring toxic catalysts, preserving antibody activity and ensuring site-specific conjugation.
20/5/2019
Dear BOC Sciences, how does the PEG4 spacer benefit DBCO-PEG4-HyNic?
The PEG4 spacer increases solubility, reduces steric hindrance, and improves flexibility between the antibody and payload. This enhances conjugation efficiency and maintains antibody binding affinity.
4/10/2017
Could you advise what type of reaction DBCO-PEG4-HyNic utilizes for payload attachment?
It utilizes strain-promoted azide-alkyne cycloaddition (SPAAC), a copper-free click chemistry reaction. This enables rapid and selective conjugation to azide-functionalized payloads under mild conditions suitable for sensitive biomolecules.
7/10/2016
Good afternoon! Which payloads are compatible with DBCO-PEG4-HyNic?
DBCO-PEG4-HyNic is compatible with azide-functionalized cytotoxic payloads, fluorophores, or imaging agents. The bioorthogonal chemistry allows efficient and reproducible ADC construction without affecting antibody function.
1/9/2019
Good afternoon! What are the detection and verification methods for DBCO-PEG4-HyNic?
DBCO-PEG4-HyNic can be analyzed using NMR, mass spectrometry, and HPLC to confirm identity and structural integrity. Proper handling under inert, dry conditions is required to prevent degradation. Aliquoting ensures the reactive HyNic moiety remains functional for consistent bioconjugation efficiency.
20/3/2017
— Dr. Jessica White, Senior Chemist (USA)
DBCO-PEG4-HyNic linker allowed efficient copper-free click chemistry, improving conjugation yields and reproducibility.
7/10/2016
— Prof. Markus Schmidt, Medicinal Chemist (Germany)
High-purity DBCO-PEG4-HyNic performed exceptionally well in our bioconjugation protocols.
20/3/2017
— Dr. Claire Dubois, ADC Development Scientist (France)
BOC Sciences delivered DBCO-PEG4-HyNic with excellent documentation and timely delivery.
1/9/2019
— Mr. Oliver Hughes, R&D Manager (UK)
The linker’s PEGylation improved solubility and reduced steric hindrance. Very reliable product.
20/5/2019
— Dr. Lina Berg, Bioconjugation Specialist (Sweden)
DBCO-PEG4-HyNic enabled smooth click reactions with minimal by-products. Highly recommended.
— Ms. Hannah Fischer, Research Scientist (Netherlands)
Excellent batch consistency of DBCO-PEG4-HyNic allowed reproducible conjugation in multiple ADC projects.
5/10/2017
DBCO-PEG4-HyNic
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DBCO-PEG4-HyNic
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