Biotin-PEG4-Dde-TAMRA-PEG3-Azide

Biotin-PEG4-Dde-TAMRA-PEG3-Azide is a multifunctional chemical probe used extensively in bioconjugation and imaging studies. Here are some key applications of Biotin-PEG4-Dde-TAMRA-PEG3-Azide:

Protein Labeling and Affinity Purification: This reagent can be used for the biotinylation and subsequent purification of proteins. The biotin moiety enables strong binding to streptavidin- or avidin-coated surfaces, facilitating the isolation of target proteins. Meanwhile, the Dde (1-(4,4-Dimethyl-2,6-dioxo-cyclohexylidene)ethyl) protection group provides a reversible option for downstream applications.

Fluorescence Microscopy: The TAMRA (Tetramethylrhodamine) fluorophore in Biotin-PEG4-Dde-TAMRA-PEG3-Azide allows for high-resolution imaging in fluorescence microscopy. Researchers use this compound to visualize cellular components in live or fixed cells. Its bright red fluorescence is particularly useful for multi-color imaging experiments.

Click Chemistry Applications: The azide group in this molecule is reactive in click chemistry, facilitating the attachment of various alkyne-modified probes or molecules. This makes it a versatile tool for introducing functional groups into biomolecules under milder conditions. Researchers can track biological processes or modify biomolecules with high precision and efficiency.

Surface Functionalization: Biotin-PEG4-Dde-TAMRA-PEG3-Azide can be employed to functionalize surfaces for biosensor development. The biotin and azide functionalities allow for versatile conjugation to various sensor substrates, improving the capture and detection of analytes. This is particularly advantageous in the creation of highly sensitive and specific diagnostic tools.