Name: Biotin-PEG2-acid
Brand: BOC Sciences
Rating: 5 (1 reviews)

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Price

Stock

Quantity

$--

In stock

Synonyms

(+)-Biotin-PEG2-acid; (+)-Biotin-PEG2-COOH; 3-(2-(2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)propanoic acid

IUPAC Name

3-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]propanoic acid

Canonical SMILES

C1C2C(C(S1)CCCCC(=O)NCCOCCOCCC(=O)O)NC(=O)N2

InChI

InChI=1S/C17H29N3O6S/c21-14(18-6-8-26-10-9-25-7-5-15(22)23)4-2-1-3-13-16-12(11-27-13)19-17(24)20-16/h12-13,16H,1-11H2,(H,18,21)(H,22,23)(H2,19,20,24)/t12-,13-,16-/m0/s1

InChIKey

SDOAIZRTYJIBBG-XEZPLFJOSA-N

Density

1.2±0.1 g/cm3

Solubility

10 mm in DMSO

Flash Point

408.7±32.9 °C

Index Of Refraction

1.527

Vapor Pressure

0.0±5.4 mmHg at 25°C

Appearance

Solid

Shelf Life

-20°C 3 years powder; -80°C 2 years in solvent

Shipping

Room temperature, or blue ice upon request.

Storage

-20°C

Boiling Point

752.2±60.0 °C at 760 mmHg

Form

Solid

Biotin-PEG2-acid, a versatile bifunctional molecule central to bioconjugation and biotechnology, finds diverse applications. Here are four key uses of Biotin-PEG2-acid presented with high perplexity and burstiness:

Protein Labeling: Employing Biotin-PEG2-acid enables precise protein labeling, facilitating detection and purification via robust streptavidin-biotin interactions. This technique proves invaluable in affinity chromatography, selectively isolating biotinylated proteins from complex mixtures. The PEG2 spacer plays a pivotal role, enhancing solubility and reducing steric hindrance, ultimately optimizing protein binding efficiency and detection accuracy.

Drug Delivery Systems: Enter the realm of targeted drug delivery systems, where Biotin-PEG2-acid emerges as a key player. By conjugating therapeutic agents to biotin, drug compounds can be guided to specific cells or tissues expressing biotin-binding proteins. The PEG2 linker takes the stage, enhancing biocompatibility and circulation time, bolstering the therapeutic efficacy of the conjugated drugs.

Immunoassays: In the realm of immunoassay development, Biotin-PEG2-acid takes center stage in biotinylating antibodies or antigens, employing streptavidin-coated surfaces for detection. This approach finds widespread use in diagnostic assays like ELISAs and western blots, owing to the high affinity and specificity of the biotin-streptavidin interaction. The PEG2 spacer serves as a critical ally, minimizing non-specific binding and elevating assay sensitivity and precision.

Cell Surface Modification: Witness the transformative potential of Biotin-PEG2-acid in cell surface modification through biotinylation of cell membrane proteins. This strategic move allows for precise attachment of streptavidin-conjugated molecules, such as fluorescent probes or nanoparticles, onto the cell surface. This technique unlocks opportunities for cell labeling, tracking, and targeted imaging studies, shedding light on intricate cell behaviors and interactions.

Catalog	Product Name	CAS
BADC-01524	Biotin-PEG1-azide	1204085-48-2
BADC-00903	NHS-SS-biotin	122266-55-1
BADC-01699	Biotin-PEG(4)-SS-Azide	1260247-52-6
BADC-01728	Biotin-PEG4-SS-Alkyne	1260247-54-8
BADC-01523	Biotin-PEG4-PFP ester	1334172-58-5
BADC-01016	PC Biotin-PEG3-alkyne	1869922-24-6
BADC-01022	PC Biotin-PEG3-azide	1937270-46-6
BADC-01672	Biotin-PEG4-MeTz	1962919-31-8
BADC-01050	PC-Biotin-PEG4-PEG3-azide	2055198-04-2
BADC-01810	Biotin-PEG3-Me-Tet	2143968-06-1

What is the primary application of Biotin-PEG2-acid in ADCs?

Biotin-PEG2-acid serves as a functional linker enabling biotinylation of antibodies or ADCs. Its PEG2 spacer enhances solubility and reduces steric hindrance, facilitating subsequent binding to avidin or streptavidin-based detection and purification systems.

23/9/2020

We are interested in how Biotin-PEG2-acid affects ADC conjugation efficiency.

The carboxylic acid group of Biotin-PEG2-acid enables efficient coupling to amino groups on antibodies using standard carbodiimide chemistry. The PEG2 spacer reduces steric interference, improving conjugation yield and reproducibility.

11/9/2021

Could you advise if Biotin-PEG2-acid is suitable for in vivo applications?

Yes, Biotin-PEG2-acid is suitable for in vivo use when applied in controlled biotin-streptavidin systems. The PEG2 spacer enhances solubility and reduces non-specific interactions, supporting targeted delivery or imaging studies.

9/6/2022

Dear BOC Sciences, which analytical techniques can verify Biotin-PEG2-acid incorporation?

Incorporation of Biotin-PEG2-acid can be confirmed by HPLC for purity, mass spectrometry for molecular weight verification, and streptavidin-binding assays to ensure functional biotin presentation on the conjugate.

1/1/2018

Dear BOC Sciences, how should Biotin-PEG2-acid be stored to preserve its functional integrity?

Biotin-PEG2-acid should be stored at low temperature in a dry, light-protected environment. Inert atmosphere or vacuum-sealed containers are recommended to prevent hydrolysis. Proper handling with gloves and protective equipment ensures stability. Supporting documents provide detailed storage guidelines.

6/4/2022

— Dr. Jonathan White, Medicinal Chemist (UK)

Biotin-PEG2-acid offered excellent biotinylation efficiency for our ADC constructs.

9/6/2022

— Ms. Anna Hoffmann, Biochemist (Germany)

High purity and solubility ensured smooth conjugation across different biomolecules.

6/4/2022

— Dr. William Parker, Senior Scientist (USA)

We achieved reproducible labeling without side reactions.

1/1/2018

— Dr. Elise Moreau, Chemist (France)

Reliable incorporation in multi-step workflows simplified our linker design.

23/9/2020

— Dr. Mark Johnson, Research Scientist (USA)

The product enabled innovative targeting strategies using biotin-streptavidin chemistry.

— Dr. Rachel Adams, Bioconjugation Scientist (USA)

Biotin-PEG2-acid from BOC Sciences enabled efficient biotinylation of our proteins. The high purity and well-documented handling instructions were very helpful.

11/9/2021