Name: Mal-PEG3-NHS ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Synonyms

Mal-PEG3-NHS; 2,5-Dioxo-1-pyrrolidinyl 3-[2-[2-[2-(2,5-dioxo-2,5-dihydro-1-pyrrolyl)ethoxy]ethoxy]ethoxy]propanoate; Propanoic acid, 3-[2-[2-[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethoxy]ethoxy]ethoxy]-, 2,5-dioxo-1-pyrrolidinyl ester; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy)ethoxy)ethoxy)propanoate; 1-{2-[2-(2-{3-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-3-oxopropoxy}ethoxy)ethoxy]ethyl}-1H-pyrrole-2,5-dione; 1H-Pyrrole-2,5-dione, 1-[2-[2-[2-[3-[(2,5-dioxo-1-pyrrolidinyl)oxy]-3-oxopropoxy]ethoxy]ethoxy]ethyl]-

IUPAC Name

(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-(2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]ethoxy]propanoate

Canonical SMILES

C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCN2C(=O)C=CC2=O

InChI

InChI=1S/C17H22N2O9/c20-13-1-2-14(21)18(13)6-8-26-10-12-27-11-9-25-7-5-17(24)28-19-15(22)3-4-16(19)23/h1-2H,3-12H2

InChIKey

IIXXEVQHRSMNOB-UHFFFAOYSA-N

Density

1.39±0.1 g/cm3 (Predicted)

Solubility

Soluble in DCM, DMSO

Appearance

Pale Yellow Oily Matter

Shipping

Room temperature, or blue ice upon request.

Storage

Store at 2-8°C

Boiling Point

552.4±60.0°C (Predicted)

Mal-PEG3-NHS ester, a versatile chemical reagent in bioconjugation with diverse applications in bioscience and biotechnology, is a key player in various domains.

Protein Modification: Through the intricate process of PEGylation, Mal-PEG3-NHS ester assumes a crucial role in the modification of proteins by the addition of polyethylene glycol (PEG) chains to their surfaces. This modification significantly boosts protein stability, solubility, and in vivo half-life, establishing itself as a cornerstone in therapeutic protein development. The selective conjugation of PEG to lysine residues on proteins via the NHS ester group allows for targeted enhancements of their properties.

Antibody Conjugation: In addition to its role in protein modification, Mal-PEG3-NHS ester facilitates the conjugation of antibodies with diverse molecules like fluorescent dyes or drug compounds, paving the way for groundbreaking diagnostic applications and precise targeted therapy. The maleimide group within this compound exhibits specificity in reacting with thiol groups, enabling site-specific labeling of antibody fragments. This tailored conjugation strategy plays a pivotal role in the evolution of antibody-drug conjugates (ADCs) and the refinement of diagnostic assays with unparalleled accuracy.

Surface Coating: Leveraging its unique properties, Mal-PEG3-NHS ester emerges as an indispensable tool for surface coating applications aimed at reducing non-specific binding and enhancing biocompatibility across a spectrum of biomedical devices. By establishing PEGylated layers on surfaces, researchers create barriers to protein adsorption and cell adhesion, a strategic approach widely embraced in the design of biosensors, microarrays, and implantable devices to optimize performance and safety through tailored modifications.

Cellular Imaging: In the domain of cellular imaging, the versatility of Mal-PEG3-NHS ester shines brightly, facilitating the conjugation of imaging probes to biomolecules for unrivaled visualization of specific proteins and cellular components. This precise labeling assists researchers in delving into dynamic cellular processes and intricate protein interactions using cutting-edge imaging modalities like fluorescence microscopy.

1.Immobilization of hydrophobic peptidic ligands to hydrophilic chromatographic matrix: a preconcentration approach

Gautam S, Loh KC.

This study presents a methodology for covalent attachment of hydrophobic peptidic ligands to hydrophilic chromatographic matrices with improved coupling efficiency. Preconcentration was introduced through the use of polyethylene glycol (PEG)-based crosslinkers. Immobilization of model hydrophobic peptide pep12 (ITLISSEGYVSS) to hydrophilic silica-amine matrix was investigated in the absence/presence of PEG-based linker. The effect of linker densities 14.2, 27.6, and 56.4 μmol/g beads on coupling efficiency was investigated. Whereas a ligand coupling efficiency of 67% was obtained in the absence of the linker, incorporating PEG-based linker at low densities allowed a 30% increase in the coupling efficiency. Although the heterobifunctional crosslinker, maleimide-PEG-NHS (N-hydroxysuccinimide) ester, can be used to couple thiol-bearing ligands to amine-functionalized matrices, no method is available for quenching free amine moieties on the matrix after ligand immobilization. The efficacy of acylating agents, acetyl chloride and oxalyl chloride, in blocking free amine groups when immobilizing the model peptide pep14 (CITLISSEGYVSSK) to silica-amine matrix using maleimide-PEG-NHS ester crosslinker was investigated. Because oxalyl chloride was nonreactive to maleimides, it allowed successful coupling of pep14 to the maleimide termini of the linkers. Adsorption studies between pep14-immobilized microspheres and human immunoglobulin M (hIgM) suggested retention of ligand activity and a 95% decrease in nonspecific binding of proteins to the matrix.

Catalog	Product Name	CAS
BADC-00442	Mal-PEG6-NHS	1137109-21-7
BADC-00448	Mal-PEG-NHS	1260092-50-9
BADC-00443	Mal-PEG5-NHS	1315355-92-0
BADC-00450	Mal-PEG4-NHS	1325208-25-0
BADC-01679	Mal-PEG2-Val-Cit-PABA-PNP	1345681-52-8
BADC-00936	Mal-PEG2-acid	1374666-32-6
BADC-00449	Mal-PEG4-PFP	1415800-42-8
BADC-00453	Mal-PEG2-NHS ester	1433997-01-3
BADC-00966	Mal-PEG4-VC-PAB-DMEA	1569261-93-3
BADC-00499	Mal-PEG6-NHS ester	1599472-25-9