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Mal-PEG2-NHS ester

  CAS No.: 1433997-01-3   Cat No.: BADC-00453   Purity: ≥98% 4.5  

Mal-PEG2-NHS ester is a non-cleavable ADC linker containing the Maleimide group, 2-unit PEG, and NHS ester.

Mal-PEG2-NHS ester

Structure of 1433997-01-3

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Category
ADC Linker
Molecular Formula
C15H18N2O8
Molecular Weight
354.31
Shipping
Room temperature
Storage
Store at -20°C under inert atmosphere

* For research and manufacturing use only. We do not sell to patients.

Size Price Stock Quantity
100 mg $629 In stock

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Popular Publications Citing BOC Sciences Products
Synonyms
2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2,5-dioxo-2H-pyrrol-1(5H)-yl)ethoxy)ethoxy)propanoate; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy)ethoxy)propanoate; Propanoic acid, 3-[2-[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethoxy]ethoxy]-, 2,5-dioxo-1-pyrrolidinyl ester; 1-[2-(2-{3-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-3-oxopropoxy}ethoxy)ethyl]-1H-pyrrole-2,5-dione; 2,5-Dioxo-1-pyrrolidinyl 3-[2-[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethoxy]ethoxy]propanoate
IUPAC Name
(2,5-dioxopyrrolidin-1-yl) 3-[2-[2-(2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]propanoate
Canonical SMILES
C1CC(=O)N(C1=O)OC(=O)CCOCCOCCN2C(=O)C=CC2=O
InChI
InChI=1S/C15H18N2O8/c18-11-1-2-12(19)16(11)6-8-24-10-9-23-7-5-15(22)25-17-13(20)3-4-14(17)21/h1-2H,3-10H2
InChIKey
KMEJZQCDHANYJV-UHFFFAOYSA-N
Density
1.42±0.1 g/cm3 (Predicted)
Solubility
Soluble in Chloroform (Slightly), DCM, Methanol (Slightly)
Appearance
Pale Yellow or Colorless Oily Matter
Shipping
Room temperature
Storage
Store at -20°C under inert atmosphere
Pictograms
Irritant
Signal Word
Warning
Boiling Point
524.3±60.0°C (Predicted)

Mal-PEG2-NHS ester is a heterobifunctional ADC linker widely employed in antibody-drug conjugate design. Featuring a maleimide group and an NHS ester, this linker enables selective conjugation between thiol-containing ADC cytotoxins and lysine residues on antibodies. The PEG2 spacer introduces hydrophilicity and flexibility, which facilitates optimal linker performance in ADC linker design by reducing steric hindrance and improving solubility, thereby enhancing the stability and pharmacokinetic profile of the resulting ADC.

In ADC payload applications, the maleimide functionality allows precise thiol-reactive conjugation, while the NHS ester reacts efficiently with primary amines on antibodies. This dual reactivity supports site-specific attachment and consistent drug-to-antibody ratios (DAR), critical parameters in developing ADCs with reproducible therapeutic efficacy. The PEG2 spacer also provides controlled distance between the antibody and cytotoxic payload, maintaining antibody-antigen binding capability and minimizing aggregation.

The chemical stability of Mal-PEG2-NHS ester under physiological conditions ensures that ADC payloads remain linked during circulation, allowing controlled delivery to target tumor cells. It is compatible with a variety of ADC cytotoxins, including microtubule inhibitors, DNA-damaging agents, and other potent payloads, supporting diverse oncology-focused bioconjugation strategies.

Mal-PEG2-NHS ester is commonly used in advanced ADC linker design, providing reliable conjugation chemistry, structural flexibility, and hydrophilicity. Its combination of precise reactivity and linker stability makes it a standard tool in constructing antibody-drug conjugates with consistent performance, enhanced solubility, and optimized therapeutic potential for targeted cancer therapy.

What is Mal-PEG2-NHS ester?

Mal-PEG2-NHS ester is a PEGylated heterobifunctional linker used in ADCs and protein conjugation. The maleimide group reacts with thiols, while the NHS ester reacts with amines, and the PEG2 spacer enhances solubility and reduces steric hindrance during conjugation.

15/8/2021

Could you please advise how Mal-PEG2-NHS ester should be stored?

It should be stored in a dry, cool, and dark environment, preferably under inert gas. Proper storage preserves the reactivity of maleimide and NHS groups, ensuring stable conjugation and reproducible experimental results.

9/7/2022

Could you kindly advise the specifications of Mal-PEG2-NHS ester?

Specifications include PEG2 length, molecular weight, reactive group ratios, and solubility. These details support experimental planning and conjugation strategy without providing specific purity data.

2/9/2020

Good afternoon! Could you kindly let me know if there are support documents for Mal-PEG2-NHS ester?

Yes, structural diagrams, handling instructions, and recommended conjugation protocols are provided. These documents assist researchers in achieving reproducible results in ADC and bioconjugation studies.

5/6/2018

Dear BOC Sciences, how is Mal-PEG2-NHS ester quality typically assessed?

Quality is assessed using NMR, LC-MS, and HPLC to confirm chemical structure, functional group integrity, and stability. These analyses ensure reliability for ADC linker applications and conjugation experiments.

25/8/2019

— Dr. Kevin Hughes, Biochemist (UK)

Mal-PEG2-NHS ester enabled highly efficient antibody modification, and the product purity was excellent.

2/9/2020

— Ms. Julia Fischer, Research Scientist (Germany)

BOC Sciences supplied Mal-PEG2-NHS ester with consistent performance, crucial for our ADC linker optimization.

25/8/2019

— Dr. Robert Clark, ADC Development Scientist (USA)

Excellent solubility and stability of Mal-PEG2-NHS ester facilitated our protein conjugation studies without unexpected aggregation.

5/6/2018

— Dr. Sophie Laurent, Medicinal Chemist (France)

Using Mal-PEG2-NHS ester, we achieved reproducible PEGylation with minimal side reactions.

15/8/2021

— Mr. Daniel Evans, Senior Scientist (Canada)

The product documentation and batch QC for Mal-PEG2-NHS ester were comprehensive, easing regulatory submissions.

— Dr. Maria Thompson, Bioconjugation Specialist (USA)

Technical guidance from BOC Sciences helped optimize Mal-PEG2-NHS ester conjugation, improving our ADC yield.

9/7/2022

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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