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DNP-PEG4-NHS ester is a PEG linker containing a DNP moiety and an NHS ester terminal group. DNP is involved in biological applications such as involvement in ion transport across membranes. The NHS ester can react with primary amines to form stable amide bonds. The hydrophilic PEG linker increases the water solubility of the compound in aqueous media.
| Catalog Number | Size | Price | Quantity | ||
|---|---|---|---|---|---|
| BADC-00692 | -- | $-- | Inquiry |
DNP-PEG4-NHS ester, a versatile reagent in biochemistry and molecular biology, finds diverse applications. Explore four key applications of DNP-PEG4-NHS ester below:
Protein Labeling: Widely adopted for protein labeling, DNP-PEG4-NHS ester effectively attaches DNP (dinitrophenyl) groups to proteins for detection or purification purposes. This compound selectively reacts with amino groups on proteins, providing precise and simple labeling. Detection of DNP-labeled proteins can be achieved using anti-DNP antibodies, facilitating a range of biochemical assays with efficacy.
Antibody Production: In the realm of monoclonal and polyclonal antibody development, DNP-PEG4-NHS ester plays a pivotal role in creating antigen conjugates. Pairing DNP with a carrier protein stimulates robust immune responses, culminating in the generation of high-affinity anti-DNP antibodies. Such antibodies serve as invaluable assets for immunoassays and various research applications, elevating the arsenal of tools available to researchers.
Surface Functionalization: Delving into surface functionalization, DNP-PEG4-NHS ester emerges as a key player in enhancing microarrays and biosensors. By introducing DNP groups onto surfaces, researchers establish precise and reactive sites for subsequent biomolecule attachment. This sophisticated technique enables the development of highly specific and sensitive diagnostic platforms.
Studying Protein Interactions: Unlocking the realm of protein-protein and protein-ligand interactions, DNP-PEG4-NHS ester facilitates insightful studies. By tagging proteins with DNP, researchers can quantify interactions with other proteins or ligands using techniques like ELISA. This strategic approach aids in unraveling intricate molecular mechanisms and identifying potential drug targets, contributing to advancements in the field of protein interaction studies.
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BOC Sciences offers comprehensive services for ADC manufacturing, including antibody modification, linker chemistry, payload conjugation, and formulation development. In particular, our payload-linker customization service offers a convenient and fast raw material channel for many ADC researchers.
BOC Sciences provides one-stop site-specific conjugation services for amino acids, glycans, unnatural amino acids, and short peptide tags. In addition, cysteine conjugation, lysine conjugation, enzymatic conjugation, thio-engineered antibody can also be obtained quickly.
BOC Sciences offers a full range of linkers, including peptide linkers, PEG linkers, click chemistry, PROTAC linkers, non-cleavable linkers, etc. We also provide custom development services for chemically labile linkers and enzymatically cleavable linkers.
