Name: BS3 Crosslinker
Brand: BOC Sciences
Rating: 5 (1 reviews)

Synonyms

Bis(sulfosuccinimidyl)suberate

IUPAC Name

1-[8-(2,5-dioxo-3-sulfopyrrolidin-1-yl)oxy-8-oxooctanoyl]oxy-2,5-dioxopyrrolidine-3-sulfonic acid

Canonical SMILES

C1C(C(=O)N(C1=O)OC(=O)CCCCCCC(=O)ON2C(=O)CC(C2=O)S(=O)(=O)O)S(=O)(=O)O

InChI

InChI=1S/C16H20N2O14S2/c19-11-7-9(33(25,26)27)15(23)17(11)31-13(21)5-3-1-2-4-6-14(22)32-18-12(20)8-10(16(18)24)34(28,29)30/h9-10H,1-8H2,(H,25,26,27)(H,28,29,30)

InChIKey

VYLDEYYOISNGST-UHFFFAOYSA-N

Solubility

10 mm in DMSO

Melting Point

>300°C

PSA

255.69000

Appearance

Light tan powder

Shipping

Room temperature

Storage

Store at -5 °C, keep in dry and avoid sunlight.

Pictograms

Irritant

Signal Word

Warning

In Vitro

Treatment of intact cells with the homobifunctional crosslinker BS3 Crosslinker produces similar amounts of covalent dimer in both normal and SAO cells. In SAO cells, copies of normal band 3 can be distinguished from SAO band 3 by treating with H2DIDS to form a crosslink between major chymotryptic fragments (60 kDa and 35 kDa) within one subunit. Successive treatment of cells with [3H]-4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonate ([3H]H2DIDS), BS3, and chymotrypsin gives 3H-labeled products that include homodimer of normal band 3 as well as products of crosslinking normal band 3 with the 60- and 35-kDa fragment of SAO band 3.

BS3 Crosslinker, a bis(sulfosuccinimidyl) suberate crosslinking reagent, is utilized widely in biochemical research for its non-cleavable and homobifunctional properties. Here are four key applications of BS3 Crosslinker:

Protein-Protein Interaction Studies: In the realm of understanding protein interactions, BS3 Crosslinker plays a pivotal role. By covalently linking interacting proteins together, researchers can stabilize transient complexes, enabling detailed analysis. This innovative technique allows for the detection of interaction partners within intricate biological mixtures, shedding light on complex protein networks.

Structural Biology: Delving into the architecture of proteins within complexes, BS3 Crosslinker is a valuable tool in structural biology. Preceding structural characterization techniques like mass spectrometry or cryo-EM, crosslinking proteins with BS3 provides essential distance constraints for precise 3D modeling. This detailed spatial information enhances our grasp of protein structure and function, offering insights into molecular mechanisms.

Cell Surface Receptor Mapping: With its unique water solubility, BS3 Crosslinker aids in mapping cell surface receptors and their interactions. Capable of permeating the cell membrane without infiltrating intracellular components, this reagent facilitates the crosslinking of surface proteins for in-depth study. By dissecting receptor complexes and signaling pathways crucial for cellular communication, BS3 Crosslinker contributes to unraveling the intricacies of cell signaling.

Antibody-Antigen Complex Analysis: In the realm of immunological studies, BS3 Crosslinker emerges as a critical tool for stabilizing antibody-antigen complexes. By crosslinking these complexes, researchers can analyze binding affinities, map epitopes, and investigate receptor-ligand interactions. This in-depth analysis is instrumental in the development of diagnostic tools and therapeutic antibodies, advancing the field of immunotherapy and precision medicine.