Name: BS3 Crosslinker disodium
Brand: BOC Sciences
Rating: 5 (1 reviews)

Synonyms

Bis(sulfosuccinimidyl) suberate sodium salt; BS3 Crosslinker; Suberate Bis(sulfosuccinimidyl) Sodium Salt; BS3 Crosslinker (disodium); BS3;bis-sulfo-succinimidyl suberate; BS3; Sodium 1,1'-(octanedioylbis(oxy))bis(2,5-dioxopyrrolidine-3-sulfonate)

IUPAC Name

disodium;1-[8-(2,5-dioxo-3-sulfonatopyrrolidin-1-yl)oxy-8-oxooctanoyl]oxy-2,5-dioxopyrrolidine-3-sulfonate

Canonical SMILES

C1C(C(=O)N(C1=O)OC(=O)CCCCCCC(=O)ON2C(=O)CC(C2=O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]

InChI

InChI=1S/C16H20N2O14S2.2Na/c19-11-7-9(33(25,26)27)15(23)17(11)31-13(21)5-3-1-2-4-6-14(22)32-18-12(20)8-10(16(18)24)34(28,29)30;;/h9-10H,1-8H2,(H,25,26,27)(H,28,29,30);;/q;2*+1/p-2

InChIKey

MGJYOHMBGJPESL-UHFFFAOYSA-L

Appearance

Off-White Solid

Quantity

Data not available, please inquire.

Storage

-20°C, protect from light; in solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

BS3 Crosslinker disodium, a homobifunctional crosslinker prevalent in protein chemistry and molecular biology research, showcases a myriad of applications. Here are four key applications intricately:

Protein-Protein Interaction Studies: Delving into the realm of protein-protein interactions, BS3 Crosslinker disodium emerges as a pivotal tool. By covalently linking proteins in close proximity, researchers can capture dynamic or enduring interactions under physiological circumstances. This methodology aids in delineating interaction networks and unraveling the intricacies of protein complex formation across diverse biological processes.

Structural Biology: Within the intricate landscape of structural biology, BS3 Crosslinker disodium serves as an indispensable agent for stabilizing protein complexes in structural explorations encompassing techniques like X-ray crystallography and cryo-electron microscopy. By crosslinking proteins prior to analysis or imaging, their native conformations are preserved, offering profound insights into their structural arrangement. This advancement facilitates the elucidation of three-dimensional protein structures, enhancing our understanding of protein assemblies.

Protein Labeling: Venturing into the domain of protein labeling, BS3 Crosslinker disodium plays a pivotal role in attaching various tags or probes to proteins. Reacting with primary amines on the target protein, this crosslinker allows for the attachment of labels such as fluorescent dyes or biotin. This modification enables the detection, quantification, and tracking of proteins across diverse experimental setups, facilitating a deeper understanding of protein dynamics.

Drug Discovery: In the realm of drug discovery, BS3 Crosslinker disodium emerges as a valuable asset for identifying and characterizing potential drug targets. By crosslinking target proteins with small-molecule inhibitors or prospective drug candidates, researchers can unveil crucial insights into binding sites and mechanisms of action. This knowledge plays a pivotal role in optimizing drug design and enhancing therapeutic efficacy, offering promising avenues for advancements in pharmaceutical research.