Actinomycin D - CAS 50-76-0

Actinomycin D - CAS 50-76-0 Catalog number: BADC-00798

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Actinomycin D is a chromopeptide antineoplastic antibiotic that exhibits high antibacterial and antitumor activity. Actinomycin D binds to single- and double-stranded DNA and subsequent inhibition of RNA and protein synthesis.

ADCs Cytotoxin
Product Name
Actinomycin D
Catalog Number
Molecular Formula
Molecular Weight
Actinomycin D

Ordering Information

Catalog Number Size Price Quantity
BADC-00798 50 mg $298
BADC-00798 100 mg $629
Actinomycin D is a chromopeptide antineoplastic antibiotic that exhibits high antibacterial and antitumor activity. Actinomycin D binds to single- and double-stranded DNA and subsequent inhibition of RNA and protein synthesis.
Actinomycin; Cosmegen; Cosmegen Lyovac; Dactinomycin; Lyovac Cosmegen
Canonical SMILES
1.42 g/cm3
Soluble in water
Melting Point
3.21 at pH 7.4
Vapor Pressure
0 mm Hg at 25 °C (est)
Mechanism Of Action
Good evidence exists that this drug bind strongly, but reversibly, to DNA, interfering with synthesis of RNA (prevention of RNA polymerase elongation) and, consequently, with protein synthesis.
Generally, the actinomycins exert an inhibitory effect on gram-positive and gram-negative bacteria and on some fungi. However, the toxic properties of the actinomycins (including dactinomycin) in relation to antibacterial activity are such as to preclude their use as antibiotics in the treatment of infectious diseases. Because the actinomycins are cytotoxic, they have an antineoplastic effect which has been demonstrated in experimental animals with various types of tumor implant. This cytotoxic action is the basis for their use in the treatment of certain types of cancer. Dactinomycin is believed to produce its cytotoxic effects by binding DNA and inhibiting RNA synthesis.
Toxicity (LD50)
In Vitro
A dramatic decrease in anti-apoptotic myeloid leukemia cell differentiation protein (Mcl-1) mRNA and protein expression was detected upon actinomycin D treatment. Further, Mcl-l over-expression caused resistance to cell death upon treatment with actinomycin D, implicating a role for the down-regulation of Mcl-1 in actinomycin D-induced apoptosis. Actinomycin D sensitized cells to ABT-737 treatment in a Bak- or Bax-dependent manner. Importantly, low concentrations of actinomycin D and ABT-737 were more effective in inducing cell death in transformed cells than their untransformed counterparts. A synergistic effect of actinomycin D and ABT-737 on cell death was observed in several human tumor cell lines. Like actinomycin D treatment, knocking down Mcl-1 expression greatly sensitized tumor cells to ABT-737, and Mcl-1 over-expression abrogated the cytotoxic effect induced by ABT-737 and actinomycin D. These results suggest that the down-regulation of Mcl-1 by actinomycin D is likely responsible for the observed synergistic effect between the two drugs.
In Vivo
In an in vivo study, the pluronic gel containing 80 nM and 80 microM actinomycin D was applied topically to surround the rat carotid adventitia; the thickness of neointima was substantially reduced (45 and 55%, respectively). The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf were all suppressed by actinomycin D. Extracellular signal-regulated kinases (Erk) involved in cell-cycle arrest were found to increase by actinomycin D.
Clinical Trial Information
NCT NumberCondition Or DiseasePhaseStart DateSponsorStatus
NCT00003688Gestational Trophoblastic TumorPhase 22013-06-10Gynecologic Oncology GroupCompleted
NCT01823315Gestational Trophoblastic DiseasePhase 32019-12-24Ding MaActive, not recruiting
NCT00075582Adult RhabdomyosarcomaPhase 32021-11-19Children's Oncology GroupCompleted
NCT04562558Gestational Trophoblastic TumorNot Applicable2021-11-16xiang yangRecruiting
NCT00900354Unspecified Childhood Solid Tumor, Protocol Specific2013-08-12Children's Cancer and Leukaemia GroupUnknown Verified June 2009 by National Cancer Institute (NCI). Recruitment status was Recruiting
Red Crystalline Powder
Shelf Life
Dilute soln are very sensitive to light /Trihydrate/;Soln should not be exposed to direct sunlight
Room temperature
Store at -20°C
Acute Toxic; Health Hazard
Signal Word
Boiling Point
1386°C at 760 mmHg

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