Name: Disuccinimidyl glutarate
Brand: BOC Sciences
Rating: 5 (1 reviews)

Synonyms

DSG Crosslinker; Di(N-succinimidyl) glutarate; Bis(2,5-dioxopyrrolidin-1-yl) glutarate

IUPAC Name

bis(2,5-dioxopyrrolidin-1-yl) pentanedioate

Canonical SMILES

C1CC(=O)N(C1=O)OC(=O)CCCC(=O)ON2C(=O)CCC2=O

InChI

InChI=1S/C13H14N2O8/c16-8-4-5-9(17)14(8)22-12(20)2-1-3-13(21)23-15-10(18)6-7-11(15)19/h1-7H2

InChIKey

LNQHREYHFRFJAU-UHFFFAOYSA-N

Density

1.5±0.1 g/cm3

Solubility

Soluble in DMF, DMSO

Flash Point

247.2±31.5 °C

Index Of Refraction

1.572

PSA

127.36000

Vapor Pressure

0.0±1.2 mmHg at 25°C

Appearance

Off-white Powder

Shipping

Room temperature

Storage

Store at -20 °C, keep in dry and avoid sunlight.

Pictograms

Irritant

Signal Word

Warning

Boiling Point

485.1±55.0 °C at 760 mmHg

Form

Solid

Biological Activity

DSG Crosslinker is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)[1] . In Vitro: ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

DSG Crosslinker, a versatile chemical reagent integral to bioconjugation and biomolecular research, finds diverse applications across various domains. Here are four key applications:

Protein-Protein Interaction Studies: A commonly utilized tool, DSG Crosslinker plays a pivotal role in stabilizing and investigating protein-protein interactions. Through covalent bonding of interacting protein pairs, researchers can seize and scrutinize transient complexes that pose challenges in conventional study methodologies. This approach illuminates the dynamic nature and functional nuances of protein assemblies entwined in diverse biological phenomena.

Structural Biology: Within the realm of structural biology, DSG Crosslinker serves as a catalyst for exploring protein structures and conformations. By strategically linking sites within a protein or between protein subunits, scientists can unveil distance constraints pivotal in modeling and validating intricate three-dimensional structures. This methodological advancement proves invaluable in unraveling the convoluted architecture of biomolecular machinery.

Antibody Conjugation: Embraced widely in antibody conjugation endeavors for diagnostic and therapeutic ventures, DSG Crosslinker facilitates the linkage of antibodies to fluorophores, enzymes, or therapeutic agents. Through this strategic conjugation, researchers and healthcare practitioners fabricate precision tools tailored for imaging, detection, or disease intervention. This precision-enhancing strategy amplifies the specificity and efficacy of diagnostic assays and therapeutic modalities significantly.

Enzyme and Receptor Studies: Instrumental in the scrutiny of enzymes and receptors, DSG Crosslinker emerges as a critical agent in stabilizing and characterizing functional complexes. By crosslinking enzymes to their substrates or receptors to their interacting ligands, researchers unearth detailed insights into binding affinities, kinetics, and mechanistic underpinnings. This profound comprehension holds pivotal significance in the realms of drug discovery and unraveling the intricate pathways underpinning biochemical cascades.