DBCO-SS-PEG4-Biotin

DBCO-SS-PEG4-Biotin is a multifunctional reagent used in bioconjugation techniques. Here are some key applications of DBCO-SS-PEG4-Biotin:

Bioconjugation: DBCO-SS-PEG4-Biotin is frequently used to label biomolecules with biotin, facilitating their subsequent capture or detection using streptavidin-based systems. Its DBCO group allows for selective and efficient conjugation with azide-modified molecules through copper-free Click Chemistry. This makes it ideal for bioorthogonal labeling in live cells or complex biological samples.

Protein Purification: The biotin moiety on DBCO-SS-PEG4-Biotin enables affinity-based purification of proteins. By tagging proteins with this reagent, they can be easily isolated using streptavidin-coated beads or columns. This method is highly effective for purifying proteins without affecting their activity or function, which is critical for downstream applications.

Drug Delivery: DBCO-SS-PEG4-Biotin can be used in the development of targeted drug delivery systems. By biotinylating drug carriers or therapeutic agents, specific targeting can be achieved through biotin-streptavidin interactions. This approach enhances the precision of drug delivery, reducing side effects and increasing efficacy.

Diagnostic Assays: In diagnostic applications, DBCO-SS-PEG4-Biotin is employed to create biotinylated probes and capture molecules. These biotin-tagged entities can bind to streptavidin-coated surfaces, facilitating the detection of target analytes in assays such as ELISA and Western blotting. The use of this reagent thus enhances sensitivity and specificity in diagnostic testing.