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(2S)-N3-HABA

  CAS No.: 959148-55-1   Cat No.: BADC-01827 4.5  

(2S)-N3-HABA is an azide-bearing amino acid linker intermediate facilitating efficient bioorthogonal conjugation for antibody-drug conjugate assembly, enhancing targeting specificity and payload stability.

(2S)-N3-HABA

Structure of 959148-55-1

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ADC Linker
Molecular Formula
C4H7N3O3
Molecular Weight
145.12

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IUPAC Name
(2S)-4-azido-2-hydroxybutanoic acid
Canonical SMILES
C(CN=[N+]=[N-])C(C(=O)O)O
InChI
InChI=1S/C4H7N3O3/c5-7-6-2-1-3(8)4(9)10/h3,8H,1-2H2,(H,9,10)/t3-/m0/s1
InChIKey
UERAIPHBLAAUFG-VKHMYHEASA-N

(2S)-N3-HABA, a compound commonly employed in protein studies and biochemical research, finds diverse applications in scientific inquiries. Here are four key applications presented with high perplexity and burstiness:

Protein-Ligand Interaction Studies: In the realm of protein-ligand interactions, (2S)-N3-HABA emerges as a pivotal tool for researchers seeking to unravel the intricate dance between proteins and ligands. By introducing (2S)-N3-HABA into experiments, scientists can scrutinize shifts in protein behavior, aiding in the pinpointing of binding sites and affinities. This application is paramount for advancing drug development efforts and shedding light on the multifaceted functions of proteins across various biological processes.

Enzyme Activity Assays: Delving into the catalytic properties of enzymes, (2S)-N3-HABA serves as a cornerstone in enzyme activity assays. Acting as both a substrate and an inhibitor in these reactions, this compound enables scientists to gauge enzymatic activity and kinetics with precision. Such insights are fundamental for unraveling enzyme mechanisms and laying the groundwork for developing enzyme inhibitors as potent therapeutic agents.

Biochemical Pathway Analysis: Within the realm of biochemical research, researchers harness the power of (2S)-N3-HABA to navigate and dissect intricate biochemical pathways. By integrating this compound into metabolic studies, scientists can track the flow of metabolites and untangle the roles of various enzymes and intermediates within these pathways. This approach aids in deciphering complex biochemical networks and optimizing applications in metabolic engineering, fostering advancements in biotechnology.

Protein Purification: In the pursuit of isolating and purifying proteins for research endeavors, (2S)-N3-HABA emerges as a key player in the realm of affinity purification. Leveraging its unique binding properties, researchers can extract and purify specific proteins from complex mixtures with precision. This application is indispensable for generating high-purity proteins essential for structural studies, functional assays, and cutting-edge pharmaceutical research initiatives.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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