Fmoc-NH-Azide-PEG4-L-Lysine-PFP ester

Fmoc-NH-Azide-PEG4-L-Lysine-PFP ester is a versatile reagent used in bioconjugation and peptide synthesis. Here are some key applications of Fmoc-NH-Azide-PEG4-L-Lysine-PFP ester:

Peptide Synthesis: Fmoc-NH-Azide-PEG4-L-Lysine-PFP ester is employed in solid-phase peptide synthesis as a building block. Its Fmoc group allows for easy removal under mild conditions, facilitating the assembly of complex peptides. This reagent ensures a high degree of purity and efficiency in the synthesis of tailored peptide sequences.

Bioconjugation: This compound is crucial in the conjugation of peptides or proteins to various molecules, such as drugs, dyes, or other biomolecules, via its azide group. The azide group enables click chemistry reactions, allowing for the creation of stable bio-conjugates with high specificity and efficiency. This application is particularly useful in drug delivery systems and diagnostic tool development.

Surface Functionalization: Fmoc-NH-Azide-PEG4-L-Lysine-PFP ester can be used to functionalize surfaces with peptides or other molecules. The PFP ester allows for strong covalent bonding to surfaces, while the PEG4 spacer provides flexibility and reduces steric hindrance. This functionalization is beneficial in creating bioactive surfaces for biosensors, cell culture substrates, and biointerfaces.

Protein Labeling: The reagent is utilized in the site-specific labeling of proteins for imaging and tracking studies. Its azide group reacts efficiently with alkyne-containing probes through click chemistry, ensuring precise labeling. This technique is widely used in fluorescence microscopy and other imaging modalities to study protein localization and dynamics.