Name: Azide-C2-SS-C2-biotin
Brand: BOC Sciences
Rating: 5 (1 reviews)

Synonyms

Azide-SS-biotin; N-(2-((2-azidoethyl)disulfanyl)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

IUPAC Name

Canonical SMILES

C1C2C(C(S1)CCCCC(=O)NCCSSCCN=[N+]=[N-])NC(=O)N2

InChI

InChI=1S/C14H24N6O2S3/c15-20-17-6-8-25-24-7-5-16-12(21)4-2-1-3-11-13-10(9-23-11)18-14(22)19-13/h10-11,13H,1-9H2,(H,16,21)(H2,18,19,22)/t10-,11-,13-/m0/s1

InChIKey

CMWHTSPPRPEKSH-GVXVVHGQSA-N

Solubility

10 mm in DMSO

Appearance

Solid

Shelf Life

0-4°C for short term (days to weeks), or -20°C for long term (months).

Shipping

Room temperature, or blue ice upon request.

Storage

Store at -20 °C, keep in dry and avoid sunlight.

Form

Solid

Azide-C2-SS-C2-biotin, a versatile reagent with diverse biochemical applications, plays a pivotal role in various realms of scientific exploration. Here are four key applications of Azide-C2-SS-C2-biotin described with heightened perplexity and burstiness:

Protein Labeling: Employing Azide-C2-SS-C2-biotin for protein biotinylation via click chemistry offers a streamlined method for protein detection and purification. This bioorthogonal process entails a precise reaction that tags proteins with biotin without impeding their native functionality. The subsequent biotin-labeled proteins can be captured using streptavidin-based systems for diverse downstream applications, including western blotting, ELISA, and affinity purification.

Cell Surface Profiling: Incorporating Azide-C2-SS-C2-biotin into cell surface molecules enables targeted labeling and analysis of cell surface proteins. This technique proves invaluable for scrutinizing cell-cell interactions, receptor dynamics, and identifying cell surface biomarkers. The biotinylated entities can be isolated and characterized using streptavidin-conjugated tools, facilitating intricate surface profiling studies.

Imaging and Localization Studies: The integration of Azide-C2-SS-C2-biotin with fluorescent streptavidin conjugates enhances imaging studies by enabling precise visualization and localization of biotinylated molecules within cellular and tissue contexts. This methodology amplifies the examination of protein distribution, intracellular trafficking patterns, and cellular architecture, offering deeper insights into cellular mechanisms.

Drug Delivery Studies: Azide-C2-SS-C2-biotin serves as a crucial component in investigating targeted drug delivery systems. By conjugating biotinylated therapeutic agents or nanoparticles with streptavidin-modified delivery vehicles, researchers can achieve specific targeting to biotin-coated surfaces or cells. This innovative approach unlocks new avenues for developing targeted therapies with heightened efficacy and minimized side effects.