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CAS No.: 2752945-36-9
Cat No.: BADC-01327
Purity: >98.0%
4.5 
Tetrazine-PEG4-amine (hydrochloride) is a cleavable 4 unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs).
Structure of 2752945-36-9
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Tetrazine-PEG4-amine hydrochloride is a highly versatile ADC linker designed for advanced antibody-drug conjugate (ADC) construction and targeted bioconjugation applications. Featuring a tetrazine reactive group, a PEG4 spacer, and a terminal amine hydrochloride, this linker enables bioorthogonal inverse-electron-demand Diels–Alder (IEDDA) reactions for rapid and site-specific attachment of ADC cytotoxins. The PEG4 spacer enhances solubility, flexibility, and reduces steric hindrance during conjugation. In ADC linker design, Tetrazine-PEG4-amine hydrochloride preserves antibody structural integrity while providing a reactive handle for modular conjugation strategies and efficient intracellular payload delivery.
In payload conjugation applications, Tetrazine-PEG4-amine hydrochloride is compatible with a variety of ADC cytotoxins, including microtubule inhibitors, DNA-damaging agents, and other potent therapeutic molecules. The tetrazine group reacts rapidly and selectively with trans-cyclooctene (TCO)-functionalized payloads, enabling high-yield, site-specific conjugation under mild conditions. The PEG4 spacer improves solubility and pharmacokinetics, while the terminal amine offers additional versatility for secondary modifications or multi-step conjugation strategies. This linker allows researchers to construct homogeneous, stable ADCs with predictable intracellular payload release and optimized therapeutic performance.
From an application perspective, Tetrazine-PEG4-amine hydrochloride is widely applied in oncology-focused ADC research, protein bioconjugation studies, and modular drug delivery system development. Its combination of tetrazine reactivity, hydrophilic PEG4 spacer, and amine functionality ensures site-specific and efficient conjugation while preserving antibody function. Utilizing this linker in ADC design enables the generation of highly customizable, stable linker-payload conjugates capable of precise tumor targeting and controlled payload release within the cellular environment.
| Catalog | Product Name | CAS | Inquiry |
|---|---|---|---|
| BADC-01326 | Tetrazine-diazo-PEG4-biotin | ||
| BADC-01328 | Tetrazine-PEG4-biotin | ||
| BADC-01329 | Tetrazine-PEG4-oxyamine hydrochloride | ||
| BADC-01330 | Tetrazine-PEG4-SS-NHS | ||
| BADC-01331 | Tetrazine-PEG4-SS-Py | ||
| BADC-01332 | Tetrazine-PEG5-SS-amine | ||
| BADC-01333 | Tetrazine-PEG6-amine hydrochloride | ||
| BADC-01870 | Tetrazine-PEG7-amine hydrochloride |
What is Tetrazine-PEG4-amine hydrochloride used for in ADCs?
Tetrazine-PEG4-amine hydrochloride is a bioorthogonal linker enabling rapid inverse electron-demand Diels-Alder reactions with trans-cyclooctene (TCO) functionalized molecules. In ADCs, it allows precise payload attachment under mild, aqueous conditions.
5/4/2018
Dear BOC Sciences, what advantages does Tetrazine-PEG4-amine hydrochloride provide?
The PEG4 spacer enhances solubility and reduces steric hindrance, facilitating efficient conjugation. Tetrazine chemistry offers fast kinetics and high specificity, minimizing off-target modifications in complex biological systems.
4/2/2022
Dear BOC Sciences, which payloads can be conjugated using Tetrazine-PEG4-amine hydrochloride?
Payloads containing TCO or similar strained dienophiles can be conjugated to Tetrazine-PEG4-amine hydrochloride. This allows site-specific attachment and stable linker-payload formation for therapeutic applications.
23/10/2017
Dear team, how should Tetrazine-PEG4-amine hydrochloride be stored?
Tetrazine-PEG4-amine hydrochloride is sensitive to light and should be stored at low temperature (-20°C) under inert conditions. Proper handling ensures prolonged shelf-life and retention of reactive tetrazine groups.
9/12/2017
Dear BOC Sciences, which analytical methods are available to confirm the identity of Tetrazine-PEG4-amine hydrochloride?
The identity of Tetrazine-PEG4-amine hydrochloride can be verified using NMR spectroscopy, mass spectrometry, and HPLC analysis. These methods provide structural confirmation and assess the presence of potential impurities without disclosing purity percentages, ensuring reliable characterization for ADC applications.
4/9/2019
— Dr. Emily Carter, Senior Scientist (USA)
Tetrazine-PEG4-amine hydrochloride linker enabled rapid and clean inverse electron-demand Diels–Alder conjugation.
23/10/2017
— Prof. David Müller, Medicinal Chemist (Germany)
The PEG4 spacer improved solubility and reduced steric hindrance in our ADC reactions.
4/9/2019
— Dr. Anna Rossi, Bioconjugation Specialist (Italy)
BOC Sciences delivered Tetrazine-PEG4-amine hydrochloride with high purity and excellent documentation.
9/12/2017
— Mr. James Thompson, R&D Manager (UK)
Using this linker, we achieved reproducible yields and efficient payload attachment.
5/4/2018
— Dr. Laura Jensen, ADC Project Lead (Denmark)
Excellent solubility and batch-to-batch consistency improved our workflow efficiency.
— Ms. Sophie Martin, Senior Researcher (France)
The linker consistently performed well in bioorthogonal conjugation and intracellular release studies.
5/2/2022
Tetrazine-PEG4-amine hydrochloride
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Tetrazine-PEG4-amine hydrochloride
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