Name: Fluorescein-DBCO
Brand: BOC Sciences
Rating: 5 (1 reviews)

Size

Price

Stock

Quantity

$--

In stock

Synonyms

DBCO-FITC(5-isomer)

IUPAC Name

Canonical SMILES

C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCNC(=S)NC4=CC5=C(C=C4)C6(C7=C(C=C(C=C7)O)OC8=C6C=CC(=C8)O)OC5=O

InChI

InChI=1S/C39H27N3O6S/c43-27-12-15-31-34(20-27)47-35-21-28(44)13-16-32(35)39(31)30-14-11-26(19-29(30)37(46)48-39)41-38(49)40-18-17-36(45)42-22-25-7-2-1-5-23(25)9-10-24-6-3-4-8-33(24)42/h1-8,11-16,19-21,43-44H,17-18,22H2,(H2,40,41,49)

InChIKey

FGEDVMJBPDYXLU-UHFFFAOYSA-N

Solubility

10 mm in DMSO

Appearance

Solid

Shelf Life

0-4°C for short term (days to weeks), or -20°C for long term (months).

Shipping

Room temperature

Storage

Store at -5°C,keep in dry and avoid sunlight.

Emission

517

Excitation

494

Form

Solid

Fluorescein-DBCO, a derivative fluorescent dye utilized in bioconjugation and imaging applications, serves a multitude of crucial roles. Here are four key applications of Fluorescein-DBCO:

Cell Imaging: Widely employed in cell imaging, Fluorescein-DBCO facilitates the labeling and visualization of biological molecules. Through a copper-free click chemistry reaction with azide-functionalized biomolecules, it enables specific and efficient tagging of target molecules within live cells. This sophisticated technique empowers scientists to monitor cellular processes in real-time, boasting high sensitivity and specificity.

Protein Labeling: In the realm of protein research, Fluorescein-DBCO offers precise protein labeling capabilities. By conjugating it to proteins featuring azide groups, researchers can track protein localization, interactions, and dynamics across diverse biological contexts. This method proves particularly valuable in studies investigating protein function, interaction networks, and cellular pathways, shedding light on the intricate workings of biological systems.

Flow Cytometry: Fluorescein-DBCO finds utility in flow cytometry for the comprehensive analysis of cell populations with unparalleled throughput and sensitivity. Following cell labeling with the dye, researchers leverage flow cytometry to measure fluorescence intensity, facilitating a detailed examination of cell surface markers, intracellular proteins, and cell viability. This technique stands as a cornerstone in fields such as immunology, cancer research, and cell biology, enabling intricate analyses crucial for understanding cellular behavior.

Biomedical Diagnostics: Within diagnostic applications, Fluorescein-DBCO plays a pivotal role in assay development for the detection of specific biomolecules. Its robust fluorescence and excellent biocompatibility make it an ideal candidate for integration into biosensors and diagnostic kits. By enabling the detection of biomolecules at low concentrations, this dye heightens the sensitivity and accuracy of diagnostic tests for a range of diseases, bolstering the capabilities of biomedical diagnostics.